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KMID : 1001020030010040371
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Korean Journal of Urological Oncology
2003 Volume.1 No. 4 p.371 ~ p.375
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Culture of Dendritic Cell from Patient with Renal Cell Carcinoma and Its Anti-tumor Immune Activity when Pulsed by Renal Cell Carcinoma Cell Line: In Vitro Study
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Jung Han
Ji Young-Hwan
Kim Choung-Soo
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Abstract
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Purpose: Dendritic cells(DC) play a crucial role in the initiation of primary immune response and are known as an excellent adjuvant for anti-cancer immunity. In this study, we tried to obtain substantial numbers of dendritic cells from peripheral blood of patients with renal cell carcinoma and normal controls. We also investigated the anti-tumor immune response of dendritic cells pulsed by renal cell carcinoma cell line A498 in vitro. We compared with two different sources of dendritic cell in view of anti-tumor activity and maturation capacity of dendritic cells
Materials and Methods: DC were generated by culturing plastic adherent mononuclear cells from the peripheral blood in the presence of granulocyte-macrophage colony-stimulating factor and interleukin-4. Immature DC were cocultured with T-cells and pulsed by A498. MTT analysis was performed using the medium in which A498 only was cultured as control.
Results: We could obtain 0.6?1.8x106 DC with phenotype typical of mature DC(CD14?, CD80?, CD83?) from the normal peripheral blood and patients with renal cell carcinoma. On T-cell proliferation assay, the number of T-cells increased in proportion to that of DC and when DC were pulsed by A498, the same phenomenon could be observed in normal control group. The number of T-cells not changed in DC from patients with renal cell carcinoma.
Conclusions: We could successfully obtain mature dendritic cells from the peripheral blood in patients with reanl cell carcinima. The data revealed indirectly that dendritic cells in patients with renal cell carcinoma treated with tumor lysate not enhance immune activity and not increases anti-cancer effect of T-cells. Further investigations including in-vivo study should be carried out to realize the effect of immunotherapy using dendritic cells against metastatic renal cell carcinoma.
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KEYWORD
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Dendritic cell, Renal cell carcinoma
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